Metabolomics responses in saliva after an acute session of high-intensity interval and continuous endurance training

Resumo

Introduction: The aim of this study was to investigate the metabolomics responses in saliva after an acute session of high-intensity interval training (HIIT) and continuous endurance training (ET). Methods: Nine young untrained men (18 to 30 years old), were underwent to three 40-min acute sessions: HIIT [5 x 4 min 90% of reserve heart rate (HRr) interspersed with 3 min at 50% FCr] and ET (70% FCr) and control session (CO) in a randomized cross-over experimental design. Saliva samples were collected before (pre) and after (post) sessions and were analyzed by H1 NMR spectroscopy to identify discriminant salivary metabolites of metabolic responses between acute sessions of HIIT, ET and CO. Multivariate statistical analyzes were applied, such as: Principal Component Analysis (PCA) to identify segregation in metabolic profile (set of metabolites) between acute training sessions for pre and post moments; Partial Least Squares Discriminat Analyzes (PLS-DA) for identification of the metabolites that best explain the total variances in the salivary metabolome; and metabolic pathway analysis by over-representation and pathway topology. The significance criterion (?) was set at 5% (P < 0.05).