Prevention of damage to the cholinergic pathway in bone marrow cells after short-term exposure to high fat diet: the effect of supplementation with omega-3 fatty acid (EPA and DHA)

Abstract

Activation of ?7nAChR receptor reduces the production of proinflammatory cytokines leading to a reduction in inflammation and an increase in survival in a mouse model of sepsis. Data from our group show that animals that consumed a short-term high fat diet (HFD) showed reduction of ?7nAChR receptor expression in bone marrow cells, so our objective is to evaluate if supplementation with omega3 polyunsaturated fatty acids can prevent the damage caused by the consumption of high fat diet in bone-marrow cells. Swiss male mice were randomly assigned to be previously supplemented with omega3 fatty acid for 17 days and consumed HFD for 3 days or HFD for 3 days without supplementation or normal chow diet, whithout supplementation. The protein content of ?7nAChR was decreased in animals fed with HFD, and there was a tendency to increase when supplemented with omega3. However, there were no significant differences between the groups. Although we do not find significant results in the basal model, omega3 supplementation may be promising in models of induced sepsis.