(1)H-NMRI metabolomic profile of skeletal muscle was altered by maternal nutritional supplementation with leucine in adult offspring tumour-bearing rats

Resumo

Cachexia is a cancer-associated metabolic syndrome that causes muscle spoliation, which leads to a reduced life quality of cancer patients. Maternal nutritional supplementation with the branched-chain amino acid leucine has been studied as an attempt to minimise/prevent the muscle waste, as an epigenetic effect from pregnancy and weaning periods during the environment modulatory role. Metabolomic analysis is an important technique to evaluate the preventive effects of maternal nutritional supplementation in a cachexia process. Thus, the present study analysed the possible regulatory effects of this supplementation on muscle metabolic profile of the adult offspring rats bearing a Walker-256 tumour. The skeletal muscle (gastrocnemius) was processed by 1H-NMRI analysis to determine the metabolomic profiles. The tumour-bearing rats were spoiled and had an increased muscle energy expenditure. The maternal leucine supplementation improved the energy availability for muscle function and activity.