Palavras-chave
Growth inhibitors
Regeneration
Como Citar
Resumo
The marine amphipod Parhyale hawaiensis is able to regenerate its appendages, limbs and tissues. The objective of this study was: (i) verify whether the regeneration of P. hawaiensis antenna can be used as an endpoint in a miniaturized toxicity test system (12 well microplates, 5 mL) and, later, (ii) apply this endpoint in the toxicity assessment of dimethyl sulfoxide (DMSO), a organic solvent commonly used in toxicity tests, and diflubenzuron (DFB), a growth regulator insecticide. The average regeneration time of fifty per cent of the population (RT50) in reconstituted saltwater tests was 16.7 days. DMSO induced the regeneration concentracions above 0.02%. As expected for chitin syntesis inhibitor, DFB strogly inhibited the antenna regeneration with a 28 d-EC50 = 0.5 ?g/L. Thefore, we conclude that regeneration time can be used as endpoint in a cost effective miniaturized toxicity test, with time < 35 d. Moreover, DMSO use is not indicate.
Referências
GHEKIERE, A. et al. Non-target effects of the insecticide methoprene on molting in the estuarine crustacean Neomysis integer (Crustacea: Mysidacea). Journal of Experimental Marine Biology and Ecology. 332. 226-234, 2006. doi: 10.1016/j.jembe.2005.11.021.
MORSLI, et al. Potential hazards of a chitin synthesis inhibitor diflubenzuron in the shrimp Penaeus kerathurus: biochemical composition of the hemolymph and muscle during the molt cycle. Advances in Environmental Biology. 9. 518-525, 2015.
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