Fluorescence-based approach for the effective development of industrial Saccharomyces cerevisiae strains

Resumo

The laboratory evolution based on sexual hybridization – haploids breeding – is a powerful approach to study and develop Saccharomyces cerevisiae strains applied to the production of renewable fuels and chemicals. In this context, we developed a simple and efficient method based on reporter proteins expression and fluorescence-activated cell sorting (FACS). Exploring the natural genetic variability of two industrial Brazilian strains we obtained haploids showing superior biomass production under tree industrially relevant stress condition. In conclusion, this approach enables the isolation of an unlimited number of recombinants haploids requiring minimal and transient engineering, providing a powerful tool for create new superior hybrids through breeding, map genes related to industrially relevant traits, improvement of commercial strains and development of new ones.