The PRP4K Kinase protein overexpression, production, purification and crystallization

Resumo

The Pre-mRNA processing factor 4B Kinase is a serine/threonine kinase responsible for the phosphorylation of essentials proteins of the tri-snRNP complex in the B Complex of the spliceosome in mammals, being that the overexpression of PRP4K has being associated with certain ovary cancer forms, Paclitaxel and Carboplatin resistance³. The understanding of the kinase domain is crucial for the ligand-based rational drug design, and our research group have being working with the obtainment of high resolution crystallographic structures of this protein domain. In this work we present some advances in the protein PRP4K kinase domain production and purification, and also the attempts of protein overexpression in mammals cells.