Characterization and study of the effect of sulfate and bicarbonate on the catalytic activity of ?-lactamase OXA-143 (P227S)
PDF

Palavras-chave

Antibiotic resistance
β-lactamase
Enzyme kinetics

Como Citar

ROSPENDOWSKI, Rafael; FAVARO, Denize; ANTUNES, Víctor. Characterization and study of the effect of sulfate and bicarbonate on the catalytic activity of ?-lactamase OXA-143 (P227S). Revista dos Trabalhos de Iniciação Científica da UNICAMP, Campinas, SP, n. 27, p. 1–1, 2019. DOI: 10.20396/revpibic2720192601. Disponível em: https://econtents.bc.unicamp.br/eventos/index.php/pibic/article/view/2601. Acesso em: 28 mar. 2024.

Resumo

The aim of this study was to determine the hydrodynamic parameters of ?-lactamase OXA-143(P227S) and to evaluate the effect of sulfate and bicarbonate salts on the penicillinase activity of the enzyme. Using chromatographic and spectroscopic techniques, it was possible to determine its diffusion coefficient, hydrodynamic radius and molecular mass, showing that mutant P227S has a tertiary structure of globular shape. The kinetic results showed an increase in catalytic activity only in the assay with increasing bicarbonate concentration. The bicarbonate supplementation also increases the thermal stability of the enzyme, which doesn't occur with the addition of sulfate.

https://doi.org/10.20396/revpibic2720192601
PDF

Referências

1Evans, B. A., Amyes, S. G., OXA ?-lactamases. Clin. Microbiol. Rev., 2014,27, 241-263.
Pinheiro, G.M.S., Ramos, C.H.I.., OXA-143, Initial characterization of newly identified mitochondrial and chloroplast small HSPs from sugarcane shows that these chaperones have different oligomerization states and substrate specificities. Plant Physiol. Biochem., 2018. 129, 285-294.

Todos os trabalhos são de acesso livre, sendo que a detenção dos direitos concedidos aos trabalhos são de propriedade da Revista dos Trabalhos de Iniciação Científica da UNICAMP.

Downloads

Não há dados estatísticos.